Trinity Blood premiered in Japan on the satellite network WOWOW on April 28, 2005 and ran until October 6, 2005. The satellite channel Animax also aired the series across its networks. The Funimation English dubbing of the series premiered on Canadian television on the Razer network on July 6, 2006; and in the United States as part of Cartoon Network's Adult Swim block on September 9, 2006.
The series enjoyed strong television ratings when it originally aired in Japan. Its North American debut was also considered successful; it is credited for being one of four Funimation properties that helped the company achieve a 47.8% sales gain in 2006.
Funimation gave Trinity Blood a 90-minute US theatrical release to promote the series. The film, titled Trinity Blood: Genesis, is a compilation of the first four episodes of the series and released on May 5, 2006 in conjunction with Anime Central in Chicago, IL.